The dentinal radicular microcrack is a difficult clinical problem to diagnose and treat, and it is one of the most common reasons for tooth extraction. These microcracks start in the radicular dentin, and laboratory studies have linked crack formation to some routine endodontic procedures, namely root canal preparation, obturation, and retreatment. Most of these studies were performed using destructive methods, such as the sectioning technique, previously developed for the study of the internal anatomy of teeth. Nowadays, technological advances in the field of imaging may lead to a more thorough understanding of dentinal microcracks. This article seeks to critically appraise the methodological aspects involved in the study of dentinal radicular microcrack formation after root canal preparation, obturation, and retreatment.